I fully endorse Bio-Rad’s CFX96 and accompanying iScript cDNA Synthesis Kit and SsoFast EvaGreen Supermix. What literally took me three full days using Invitrogen’s kits took me FOUR HOURS using Bio-Rad’s equipment and reagents. The PCR efficiencies are ~20% better than SYBR Green Supermixes, Ct values are lower when running a head-to-head comparison (even handicapping the Bio-Rad reagents), run times are less than 40 minutes, and the data is cleaner! I started and completed a project in one afternoon. Fucking incredible.