Ultranerd post – feel free to skip

I fully endorse Bio-Rad’s CFX96 and accompanying iScript cDNA Synthesis Kit and SsoFast EvaGreen Supermix.  What literally took me three full days using Invitrogen’s kits took me FOUR HOURS using Bio-Rad’s equipment and reagents.  The PCR efficiencies are ~20% better than SYBR Green Supermixes, Ct values are lower when running a head-to-head comparison (even handicapping the Bio-Rad reagents), run times are less than 40 minutes, and the data is cleaner!  I started and completed a project in one afternoon.  Fucking incredible.

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Filed under animal research, grad school, life, Those Other Sciences

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